| 发明名称 |
Cloning and producing the N.BstNBI nicking endonuclease |
| 摘要 |
The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.
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| 申请公布号 |
US6191267(B1) |
申请公布日期 |
2001.02.20 |
| 申请号 |
US20000586935 |
申请日期 |
2000.06.02 |
| 申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
KONG HUIMIN;HIGGINS LAUREN S.;DALTON MICHAEL;KUCERA REBECCA B.;SCHILDKRAUT IRA |
| 分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/16;C12N9/22;C12P21/02;(IPC1-7):C07H21/04;C12N15/85;C12N15/86;C12N1/20;C12N15/63;C12N15/00 |
主分类号 |
C12N15/09 |
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