Method for obtaining dendritic cells, resulting dendritic cells and uses thereof for clinical purposes

摘要

The invention concerns a method for obtaining dendritic cells which consists in: 1) culturing, during 4 to 6 days, preferably 5 days, mononucleate cells derived from cytapheresis after mobilisation, in a serum-free medium with added human albumen in the presence of a granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin (IL) blocking the differentiation towards the macrophage path; 2) adding to the culture medium TNF- alpha and optionally an inflammatory mediator and proceeding with the culture during 1 to 4 days, preferably 2 more days; 3) recuperating the resulting dendritic cells. Said cells are useful in immunotherapy.