METHOD OF PREPARING ALPHA-PHETOPROTEIN

摘要

medicine and biotechnology for preparation of human alpha-phetoprotein. SUBSTANCE: affinic resin containing at least 1 g/l of antibodies to alpha phetoprotein is mixed with four-fold diluted blood serum of cattle and the whole is kept under stirring. Liquid is decanted, and four-fold diluted abortive human. Serum is added to resin, mixture is kept under stirring, resin is transferred into chromatographic column where it is washed with 1.5 M solution of magnesium chloride. The resulting solution is subjected to ultrafiltration on PA-50 fibers till saline composition is replaced by physiological solution to prepare concentrate containing at least 90% of human alpha-phetoprotein agent, polysaccharide is added thereto and packed into ampoules and lyophilic drying is carried out. EFFECT: simpler and cheaper method of high purity and quality of the resulting products. 3 cl