| 摘要 |
PROBLEM TO BE SOLVED: To assay an HIV-1p24 antigen with high sensitivity using one kind of polyclonal antibody for recognizing an HIV-1p24 antigen and two kinds of monoclonal antibody. SOLUTION: A polyclonal antibody having a main recognition part of HIV-1p24 antigen is employed and an HIV-1p24 antigen is purified as an immunogen. The polyclonal antibody preferably recognizes the C end region of HIV-1p24 antigen, especially recognizes peptide p24bc (listed at sequence number 24) of amino acid sequence 113-303. Main recognition part of monoclonal antibody is difference from that of polyclonal antibody and one kind thereof recognizes the N or end peptide p24a + (amino acid sequence listed at sequence number 28), i.e., amino acid sequence 1-112 of HIV-1p24 antigen, and produces N end peptide as immunogen. The other kind thereof recognizes an intermediate part or peptide p24ab (listed at sequence number 20), i.e., amino acid sequence 22-212, and produces peptide at the intermediate part as immunogen.
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