Disclosed are novel coumarin based fluorogenic compounds useful in assaying for biological activity. Specifically, these fluorogenic compounds exhibit fluorescence at particular wavelengths when cleaved by target enzymes. Preferred compounds include sugar and peptide derivatives of umbelliferone derivatives bearing a heterocyclic five membered ring at the 3-position. These compounds can be used for rapidly detecting food pathogens and for determining sterilization effectiveness. The compounds may also be used in a form bounded to a polymeric support or to a biomolecule or macromolecule.
申请公布号
WO0110881(A1)
申请公布日期
2001.02.15
申请号
WO2000US20715
申请日期
2000.07.28
申请人
3M INNOVATIVE PROPERTIES COMPANY
发明人
BENTSEN, JAMES, G.;MICKLESON, CHRISTOPHER, A.;KNUDSON, ORLIN, B.;LEWANDOWSKI, KEVIN, M.