发明名称 |
THE ISOLATION AND ENRICHMENT OF NEURAL STEM CELLS FROM UNCULTURED TISSUE BASED ON CELL-SURFACE MARKER EXPRESSION |
摘要 |
The invention provides methods for the prospective identification, isolation, and self-renewal of neural stem cells from the mammalian peripheral nervous system and compositions of neural stem cells derived from uncultured tissue. Using flow-cytometry, neural crest derived cells of embryonic peripheral nerve were fractionated based on cell surface markers. The isolated p75<+> P0 cells from embryonic sciatic nerve were phenotypically and functionally indistinguishable from neural crest stem cells (NCSCs) previously isolated from neural tube explant cultures. Furthermore, freshly isolated p75<+> P0 cells gave rise to both neurons and glia when transplanted in vivo. Cell cycle analysis and BrdU labeling indicated that p75<+>P0 NCSCs persist in the peripheral nerve by undergoing self-renewing divisions after neural crest migration has ceased.
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申请公布号 |
WO0052143(A3) |
申请公布日期 |
2001.02.15 |
申请号 |
WO2000US05840 |
申请日期 |
2000.03.03 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY;ANDERSON, DAVID, J.;MORRISON, SEAN |
发明人 |
ANDERSON, DAVID, J.;MORRISON, SEAN |
分类号 |
A61K35/12;C12N5/02;C12N5/0797;(IPC1-7):C12N5/02;C12N5/06;C12N5/08 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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