| 摘要 |
A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization condition allows the capture probe and the target polynucleotide to hybridize, and the second hybridization condition allows the capture probe and the immobilized probe to hybridize, to produce a complex of immobilized probe, capture probe and target polynucleotide. A method for determining the presence of a target polynucleotide in a sample by capturing a target polynucleotide, amplifying the captured target polynucleotide and detecting amplified sequences is also disclosed.
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