| 摘要 |
<p>A method is disclosed for generating cDNA libraries which are enhanced for 5' sequences. First strand cDNA synthesis is initiated with oligonucleotide primers which comprise, 5' to 3', 1) a defined sequence which can be used as a PCR primer and, optionally, which contains at least a portion or one or more restriction enzyme site(s), and 2) a random oligonucleotide sequence. CDNAs which have copied their mRNA templates substantially to the 5' ends are preferentially selected, optionally amplified by PCR, and cloned directionally into a vector. A novel cloning vector is described which allows for the selection of cDNAs that encode signal sequences.</p> |
