发明名称 |
METHODS AND APPARATUS FOR TEMPLATE CAPTURE AND NORMALIZATION FOR SUBMICROLITER REACTION |
摘要 |
Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided. |
申请公布号 |
WO0109389(A2) |
申请公布日期 |
2001.02.08 |
申请号 |
WO2000US21182 |
申请日期 |
2000.08.02 |
申请人 |
MOLECULAR DYNAMICS, INC. |
发明人 |
HADD, ANDY;JOVANOVICH, STEVAN |
分类号 |
C12N15/09;B01D57/02;B01J4/00;B01J19/00;B01J19/26;B01L3/00;B01L7/00;B01L99/00;B03C5/00;C12M1/00;C12M1/34;C12M1/38;C12Q1/68;C40B40/02;C40B40/06;C40B50/06;C40B60/04;C40B60/14;G01N1/00;G01N27/447;G01N31/20;G01N33/50;G01N35/00;G01N35/02;G01N35/10;G01N37/00;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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