| 摘要 |
An antimicrobial protein or peptide derived from a plant defensin, or a derivative thereof, characterised in that said protein or peptide comprises one or more of the following replacement amino acid residues selected from t he group consisting of: (i) a tryptophan residue at position 32; (ii) a valine, leucine, isoleucine, tryptophan, phenylalanine, lysine, arginine, tyrosine, methionine, cysteine or histidine residue at position 34; (iii) an isoleucin e, tryptophan, lysine, arginine, valine, leucine, phenylalanine or histidine residue at position 35; (iv) a tryptophan residue at position 36; (v) a tryptophan, glycine, threonine, tyrosine, glutamine, lysine, arginine, phenylalanine or histidine residue at position 37; (vi) a leucine, isoleucin e, tryptophan, phenylalanine, valine or cysteine residue at position 38; (vii) a leucine, isoleucine, tryptophan, phenylalanine, methionine, lysine, arginine , tyrosine or histidine residue at position 39; (viii) a tryptophan residue at position 40; (ix) an isoleucine, a tryptophan, phenylalanine, serine, threonine, tyrosine, glutamine, asparagine, lysine, arginine, histidine at position 41; and/or (x) a valine, leucine, isoleucine, tryptophan, phenylalanine, tyrosine, asparagine, lysine, arginine, serine or threonine residue at position 42; where saidamino acid residues are not found naturall y at said positions in the antimicrobial protein or peptide, with the proviso that the antimicrobial proteins do not comprise only a replacement arginine residue at position 37, 39 or 42. These proteins or peptides are useful in agriculture and pharmaceuticals.
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