HUMAN P53 MUTATIONS AND A GENETIC SYSTEM IN YEAST FOR FUNCTIONAL INDENTIFICATION OF HUMAN P53 MUTATIONS

摘要

The present invention provides isolated polypeptides of human p53 that conta in mutations. These mutations can be toxic mutations, supertransactivating mutations or tox-suppressor mutations. Further provided by the invention are methods of identifying toxic, supertransactivating, weak transactivating and tox-suppressor mutations as well as methods of identifying compounds that mimic the toxic, supertransactivating and tox-suppressor mutations in human p53. Also provided are methods of inducing toxicity in a cell by administeri ng a polypeptide comprising a supertransactivating or a toxic mutation.