发明名称 |
METHOD FOR GENERATING RESISTANCE AGAINST CGMMV IN PLANTS, GENETIC CONSTRUCTS FOR USE IN SAID METHOD, AND CGMMV-RESISTANT PLANTS OBTAINED VIA SAID METHOD |
摘要 |
The present invention relates to a method for generating resistance against Cucumber Green Mottle Mosaic Virus (CGMMV) in plants, in particular in plants susceptible to infection by CGMMV, such as <i>Cucurbitaceae</i> species, including melon, cucumber, watermelon and bottlegourd. In the invention, the plants are provided with replicase-mediated resistance against CGMMV. For this purpose, the plants are transformed with a polynucleotide sequence that: i) has been derived from the 129 kD sequence, the 57 kD sequence, or the 186 kD readthrough sequence of native CGMMV; ii) upon (at least) transformation into the plant and transcription into RNA - and usually also translation into the corresponding encoded protein - can provide the plant with resistance against CGMMV; but iii) does not encode any replicase activity. In particular, this will be a nucleotide sequence that encodes a defective variant of the replicase of CGMMV. The invention further relates to genetic constructs containing such a polynucleotide sequence suitable for use in the above method, and to CGMMV-resistant transgenic plants obtained via said method.
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申请公布号 |
WO0109300(A2) |
申请公布日期 |
2001.02.08 |
申请号 |
WO2000NL00534 |
申请日期 |
2000.07.27 |
申请人 |
KEYGENE N.V.;FIERENS-ONSTENK, BERNARDA, GERHARDA, JOHANNA;DE BOTH, MICHIEL, THEODOOR, JAN |
发明人 |
FIERENS-ONSTENK, BERNARDA, GERHARDA, JOHANNA;DE BOTH, MICHIEL, THEODOOR, JAN |
分类号 |
A01H5/00;C07K14/08;C12N1/21;C12N9/12;C12N15/40;C12N15/54;C12N15/82;(IPC1-7):C12N15/00 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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