| 摘要 |
<p>Method for analyzing a phyletic lineage of scallop, which comprises the steps of sequencing a mitochondrial DNA of the scallop to determine a nucleotide base sequence of the mitochondrial DNA, wherein the nucleotide base sequence includes a non-coding region containing a nucleotide base substitution locus which shows a sequence polymorphism indicative of a particular lineage of the scallop. Mitochondrial DNA of the scallop is amplified by PCR, using a suitable primer set designed on the basis of nucleotide base sequences conserved among known shellfish mitochondrial 16S rRNA and 12S rRNA genes, followed by sequencing to determine a non-coding region therein. In such non-coding region, a nucleotide base substitution locus is located, whereby a particular lineage of the scallop is determined. Based on those steps, a Japanese scallop, Patinopecten yessoensis, is analyzed as to the nucleotide base sequence and lineage thereof.</p> |
