发明名称 |
Intron-based assay for detecting and characterizing chromosomal rearrangement |
摘要 |
An assay is provided for detecting and characterizing chromosomal rearrangements in eukaryotic cells by genetically engineering a eukaryotic cell to contain a selectable/counter-selectable marker gene with a site for generating a double strand break within an intron of the eukaryotic cell; generating the double strand break; detecting cells which repair the double strand break by chromosomal rearrangements; isolating DNA from the detected cells; and characterizing the chromosomal rearrangement as a nonhomologous translocation, inversion, deletion or insertion.
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申请公布号 |
US6183969(B1) |
申请公布日期 |
2001.02.06 |
申请号 |
US19990293569 |
申请日期 |
1999.04.15 |
申请人 |
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY |
发明人 |
GABRIEL ABRAM |
分类号 |
C12N15/10;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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