METHOD FOR MASS-PRODUCING RECOMBINANT PROTEIN BY NOVEL EXPRESSION SYSTEM COMPRISING FUSION EXPRESSION VECTOR OF POLYHYDRIN PROMOTOR OF AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS ( ACNPV) AND PROMOTOR OF GP64

摘要

PURPOSE: Provided are Baculovirus expression vector comprising a fusion promotor wherein a polyhydrin promotor of Autographa Californica nuclear polyhedrosis virus (AcNPV) and a promotor of envelope protein glycoprotein gp64 are fused together and signal sequence of gp64 and foreign genetic sequence, and a method for mass-producing a recombinant protein. The Baculovirus vector enables separation and purification of a useful foreign protein as an active form from the cell culture and to increase the expression quantity. CONSTITUTION: The Baculovirus expression vector, pPGP404, includes a fusion promotor wherein polyhydrin promotor including nucleotide sequence of SEQ ID NO:9 originated from Autographa Californica nuclear polyhedrosis virus (AcNPV) and a promotor of envelope protein glycoprotein gp64 including 1-292 bases of nucleotide sequence of SEQ ID NO:10 are fused together. E.coli transformant, TOP10F'/pPGP404(KFCC-11031) is prepared by transforming E.coli TOP10F' strain with the expression vector pPGP404.