发明名称 METHOD AND APPARATUS FOR THE SEPARATION OF PARTICLES ESPECIALLY OF MACROMOLECLES BY ELECTROPHORESIS
摘要 The invention relates to a method for the electrophoretic separation of particles, especially of macromolecules. The inventive method comprises the following steps: applying the particles to be separated on a substrate- supported membrane so that the particles are mobile across the surface of th e substrate-supported membrane, supplying an electrical field in such a manner that electrical fields are formed across the surfaces across which the particles are mobile, temporarily modifying the electrical fields and/or usi ng a substrate-supported membrane with a structured surface, the direction and strength of the electrical field being temporarily modified such and/or the substrate-supported membrane being structured such that a force is acting on the particles that leads to a movement that depends on the length of the particles. The invention further provides a substrate especially for supporting a membrane that consists of an optically transparent material and that is used in the inventive method. The invention is also characterized by a substrate-supported membrane for carrying out the inventive method with an inventive substrate according to any of the claims, and a fluid lipid membrane. The invention provides a micro-channel electrophoresis chamber wit h at least one channel the bottom of which comprises an inventive substrate an d with an electrode system.
申请公布号 CA2386437(A1) 申请公布日期 2001.02.01
申请号 CA20002386437 申请日期 2000.07.26
申请人 RADLER, JOACHIM;MAIER, BERENIKE;ZANTL, ROMAN;RADLER, ULF;NISSEN, JULIA;KAHL, JOHAN-VALENTIN 发明人 RADLER, JOACHIM;MAIER, BERENIKE;ZANTL, ROMAN;RADLER, ULF;NISSEN, JULIA;KAHL, JOHAN-VALENTIN;GALNEDER, REINHARD;HOHNER, ANDREAS
分类号 B01D57/02;G01N27/447;(IPC1-7):G01N27/447 主分类号 B01D57/02
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