发明名称 |
ANTI-GROWTH FACTOR RECEPTOR AVIDIN FUSION PROTEINS AS UNIVERSAL VECTORS FOR DRUG DELIVERY |
摘要 |
A fusion protein for delivery of a wide variety of agents to a cell via antibody-receptor-mediated endocytosis comprises a first segment and a second segment: the first segment comprising a variable region of an antibody that recognizes an antigen on the surface of a cell that after binding to the variable region of the antibody undergoes antibody-receptor-mediated endocytosis, and, optionally, further comprises at least one domain of a constant region of an antibody; and the second segment comprising a protein domain selected from the group consisting of avidin, an avidin mutein, a chemically modified avidin derivative, streptavidin, a streptavidin mutein, and a chemically modified streptavidin derivative. Typically, the antigen is a protein. Typically, the protein antigen on the surface of the cell is a receptor such as a transferrin receptor or an insulin receptor. The invention also includes an antibody construct incorporating the fusion protein that is either a heavy chain or a light chain together with a complementary light chain or heavy chain to form an intact antibody molecule. The invention further includes targeting methods and screening methods.
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申请公布号 |
WO0107084(A1) |
申请公布日期 |
2001.02.01 |
申请号 |
WO2000US19827 |
申请日期 |
2000.07.21 |
申请人 |
REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
MORRISON, SHERIE, L.;PENICHET, MANUEL, L.;COLOMA, JOSEPHINA, M.;PARDRIDGE, WILLIAM, M.;SHIN, SEUGN-UON;NG, PATRICK, P. |
分类号 |
C07K14/465;C07K16/28;C12N15/87;(IPC1-7):A61K39/44;C12N15/09;C12N15/11;C12N15/62;C07K17/00 |
主分类号 |
C07K14/465 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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