发明名称 |
TRANSPOSON MEDIATED DIFFERENTIAL HYBRIDISATION |
摘要 |
A method for identifying an essential gene of an organism comprises: (i) providing a library of transposon mutants of the said organism; (ii) isolati ng polynucleotide sequences from the library which flank inserted transposons; (iii) hybridising the said polynucleotide sequences with a polynucleotide library from the said organism; and (iv) identifying a polynucleotide in the said polynucleotide library to which the said polynucleotide sequences do no t hybridise, thereby to determine an essential gene of the organism.
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申请公布号 |
CA2380171(A1) |
申请公布日期 |
2001.02.01 |
申请号 |
CA20002380171 |
申请日期 |
2000.07.26 |
申请人 |
ARROW THERAPEUTICS LIMITED |
发明人 |
CHARLES, IAN GEORGE;MASKELL, DUNCAN JOHN |
分类号 |
C12N15/09;A61K39/02;A61K45/00;A61P31/04;A61P31/10;A61P33/00;C12N1/21;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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