| 摘要 |
An agent (A) for prophylaxis and treatment of tumors comprising a vector (I), an enhancer-promoter (II) and a transgene (III), with >= 1 of these components being able to enter normal cells, is new. An independent claim is also included for a similar agent (A') containing an antitumor transgene (IIIa), or its sequences, provided with a membrane anchor sequence. - ACTIVITY : Cytostatic. - MECHANISM OF ACTION : Inhibition of enzymes that break down extracellular matrix, increasing collagen content of tissue, or improving cell-cell and cell-matrix adhesion, all of which lead to reduced metastatic invasion. Liver metastases were induced in mice by injecting 2 million LS174 cells into the spleen, 3 days after administration of 3X1010> plaque-forming units of an adenoviral vector expressing the TIMP-2 metalloproteinase inhibitor. After 4 weeks, the mean weight of liver tumors was 200 mg, compared with 3200 mg in untreated controls. |
