发明名称 |
THERMOSTABLE NUCLEOSIDE DIPHOSPHATE KINASE FOR NUCLEIC ACID DETECTION |
摘要 |
A thermostable nucleoside diphosphate kinase (NDPK) enzyme useful in a process for the detection of nucleic acid is disclosed. The enzyme, its variants and analogs exhibit higher NDPK activity at a temperature of about 50 DEG C to about 90 DEG C relative to NDPK activity at 37 DEG C. Methods of obtaining, preparing and using the enzyme are also disclosed. |
申请公布号 |
WO0107580(A1) |
申请公布日期 |
2001.02.01 |
申请号 |
WO2000US04206 |
申请日期 |
2000.02.18 |
申请人 |
PROMEGA CORPORATION |
发明人 |
ANDREWS, CHRISTINE, ANN;HARTNETT, JAMES, R. |
分类号 |
G01N33/53;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/12;C12N15/09;C12N15/54;C12Q1/04;C12Q1/48;C12Q1/66;C12Q1/68;C12R1/01;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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