摘要 |
PURPOSE:To efficiently produce large amounts of crocin and crocetin which are pigments of saffron by culturing saffron pistil in a medium containing specific compounds such as salicylic acid (ester) and benzoic acid (ester). CONSTITUTION:Tissue culture of saffron pistil is carried out by using a medium containing preferably 10-100ppm (based on the medium) of a compound selected from salicylic acid, a salicylic acid ester, benzoic acid, a benzoic acid ester and acetylsalicylic acid. The culture is preferably carried out at 20-25 deg.C in a dark place. The medium is e.g. Murashige-Skoog medium. |