发明名称 |
METHOD FOR SYNTHESIZING DNA |
摘要 |
A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3 @ -> 5 @ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3 @ -> 5 @ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3 @ -> 5 @ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction. |
申请公布号 |
EP1072678(A1) |
申请公布日期 |
2001.01.31 |
申请号 |
EP19990917081 |
申请日期 |
1999.04.21 |
申请人 |
TAKARA SHUZO CO, LTD. |
发明人 |
ASADA, KIYOZO;UEMORI, TAKASHI;SATO, YOSHIMI;FUJITA, TOMOKO;MIYAKE, KAZUE;TAKEDA, OSAMU;MUKAI, HIROYUKI;KATO, IKUNOSHIN |
分类号 |
C12N15/10;C12N9/12;C12N9/96;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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