| 摘要 |
The present invention concerns compositions and methods for DNA sequencing and SNP detection. The methods comprise use of ligation reactions, using one or more primers, sets of n-mer oligonucleotides and n-mer terminators. DNA sequence information is generated by analysis of the ligation products of primers, n-mers and n-mer terminators. Use of multiple primer reactions allows the generation of sequence information with higher throughput, while retaining the longer read-length that is obtained by avoiding the requirement for single-base resolution of DNA sequencing reaction products. Use of specially designed primer(s) and n-mer oligonucleotide(s) allows the generation of ligation reaction products corresponding to SNP(s). Identification of the ligation reaction products allows the determination of the SNP(s). |
