FLUOROIMMUNOASSAY AND DEVICE THEREFOR

摘要

PROBLEM TO BE SOLVED: To measure the antigen concentration with high sensitivity by selectively exciting only a fluorescent labeled antibody by use of a surface plasmon resonance. SOLUTION: A triangular glass prism 10 having a metallic thin film 11 with an antibody fixed thereon is placed on a rotating table 1 and a sample solution containing an antigen is injected into a flow cell 13 and supplied to the metallic thin film 11 to cause the antigen to bond to the antibody. Further, a fluorescent labeling reagent containing the fluorescent labeled antibody is supplied to cause the fluorescent labeled antibody to bond to the antibody bonded to the antigen. Next, light that is normally absorbed by the fluorescent labeled antibody and light whose wavelength is an integer multiple of the former light are made to impinge on the prism side of the triangular glass prism 10 at the same time to cause two-photon excitation or multi-photon excitation of the fluorescent labeled antibody; fluorescence generated when the light impinges on the prism side of the triangular glass prism with an impinging angle at which reflectivity is lowest is subjected to spectrum analysis.