| 摘要 |
PROBLEM TO BE SOLVED: To provide a pretreatment method for easily and accurately measuring a protein-coupled saccharified protein in a living body. SOLUTION: For this simple and quick pretreatment method, a proteolytic enzyme such as pronase or protinase-K is used, and an enzyme reaction is effected at a relatively high temperature (about 550 deg.C) so as to reduce the reaction time. In enzyme immunoassay, a pretreatment reagent is used in deactivating residual enzyme activity that may cause damage to an antibody and an antigen, by boiling in boiled water for about ten and several minutes. In the method, the time required to bring about a protein decomposition reaction by means of an enzyme and deactivate the enzyme is two hours or less, and a sample pretreated by the simple method in a short time can be put to precise analysis by anzyme immunoassay.
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