发明名称 |
A METHOD FOR THE ENRICHMENT OF HETERODUPLEXES AND ITS USE IN MUTATION DETECTION |
摘要 |
A method for selectively enriching heteroduplexes from a mixture of hetero- and homoduplexes comprising binding selectively said heteroduplexes to a heteroduplex binding protein thereby forming a complex between the heteroduplex and the protein. The method is characterized in that the protein is a modified form of a wild-type resolvase, which modified form in comparison to the wild type resolvase retains an ability to selectively bind to heteroduplexes, and has a modified ability to cleave heteroduplexes. In case one of the nucleic strands in the heteroduplexes derives from a sample and the other is a standard nucleic acid strand, the method may be used for detecting mismatches in the sample nucleic acid relative the standard nucleic acid.
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申请公布号 |
WO0105800(A2) |
申请公布日期 |
2001.01.25 |
申请号 |
WO2000EP06829 |
申请日期 |
2000.07.18 |
申请人 |
KEMPER, BORRIES;GOLZ, STEFAN |
发明人 |
KEMPER, BORRIES;GOLZ, STEFAN |
分类号 |
C12Q1/68;(IPC1-7):C07H/ |
主分类号 |
C12Q1/68 |
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