| 摘要 |
<p>A recombinant adenovirus vector comprising an adenovirus genomic DNA and an expression cassette is produced by inserting and ligating a cosmid sequence having recombinase recognition sequences at both ends and the expression cassette into either deletion sites of the adenovirus genomic DNA with the deletion of the E1 domain or E1 and E3 domains to thereby construct a recombinant cosmid adenovirus vector; cotransfecting this recombinant adenovirus vector and a recombinase expression vector into a cell producing adenovirus E1 protein; and eliminating the cosmid vector sequence from the recombinant cosmid adenovirus vector in the cell. This method makes it possible to conveniently and efficiently construct a recombinant adenovirus vector.</p> |
