GENERATION OF STABLE DIMERS WITH REVERSIBLE BIOTIN-BINDING ACTIVITY
摘要
Avidin is mutated by replacing the Trp110 with Lys, and streptavidin is mutated by replacing the Trp120 with Lys. Both of these mutant proteins produce a stable dimer. These stable dimers exhibit reversible biotin-binding properties. The mutant avidin and streptavidin can be used in all known avidin/streptavidin-biotin systems and purification processes, with the added advantage that the avidin/streptavidin and biotin may be eventually separated.
申请公布号
WO0105977(A1)
申请公布日期
2001.01.25
申请号
WO2000US18959
申请日期
2000.07.13
申请人
YEDA RESEARCH AND DEVELOPMENT CO. LTD.;KULOMAA, MARKKU, S.;BAYER, EDWARD, A.;WILCHEK, MEIR;LAITINEN, OLLI, H.
发明人
KULOMAA, MARKKU, S.;BAYER, EDWARD, A.;WILCHEK, MEIR;LAITINEN, OLLI, H.