摘要 |
<p>A novel protein and a process of producing the protein is provided. The protein is a glycoprotein having activity of suppressing the differentiation and/or maturation of adipocyte, having a molecular weight of about 45 kD under non-reducing conditions and about 28 kD and/or 23 kD under reducing conditions, and exhibiting affinity to heparin. A process of producing the protein comprising culturing human fibroblasts and purifying the culture broth by chromatography using an ion exchange column, affinity column, and reverse phase column. A cDNA encoding the protein and a process of producing the protein using the cDNA are also provided. The protein of the present invention is useful as a pharmaceutical composition for preventing or treating obesity or as an antigen for establishing immunological diagnosis, etc..</p> |
申请人 |
SNOW BRAND MILK PRODUCTS, CO., LTD. |
发明人 |
GOTO, MASAAKI;TOMOYASU, AKIHIRO;YANO, KAZUKI;KOBAYASHI, FUMIE;NAKAGAWA, NOBUAKI;YASUDA, HISATAKA;YAMAGUCHI, KYOJI;KINOSAKI, MASAHIKO;MOCHIZUKI, SHIN-ICHI;NAKAKARUMAI, TADASHI;MORINAGA, TOMONORI;TSUDA, EISUKE;HIGASHIO, KANJI |