发明名称 METHOD OF PREPARING COLLAGEN FROM OSSEOUS TISSUE
摘要 medicine. SUBSTANCE: osseous crumbs (size is 700-2000 mcm) are hydrolyzed in a mixture of 0.4 N sodium hydroxide and ethanol taken in the ratio 1: 1 for 24-48 h, neutralized to pH 6.8-7.7, centrifuged at 3000-5000 rev/min for 20-40 min and supernatant is discarded. Precipitate is treated with 0.125% papain solution at 37 C and then hydrolyzed again with a mixture of 0.4 N sodium hydroxide and ethanol in the ratio 1:1, dialyzed against water, frozen and lyophilized. Invention can be used for preparing medicinal forms and making medicinal articles used in general surgery, stomatology and dermatology. EFFECT: improved quality of end product. 1 cl, 1 ex
申请公布号 RU2161976(C1) 申请公布日期 2001.01.20
申请号 RU20000109783 申请日期 2000.04.21
申请人 PANASJUK ANDREJ FEDOROVICH;IVANOV SERGEJ JUR'EVICH;LARIONOV EVGENIJ VIKTOROVICH;LEVIN VADIM OLEGOVICH;SAVASHCHUK DMITRIJ ALEKSEEVICH 发明人 PANASJUK A.F.;IVANOV S.JU.;LARIONOV E.V.;LEVIN V.O.;SAVASHCHUK D.A.
分类号 A61K35/32;(IPC1-7):A61K35/32 主分类号 A61K35/32
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