发明名称 RECOMBINANT PARAINFLUENZA VIRUS VACCINES ATTENUATED BY DELETION OR ABLATION OF A NON-ESSENTIAL GENE
摘要 Recombinant parainfluenza virus (PIV) are provided in which expression of th e C, D and/or V translational open reading frame(s) (ORFs) is reduced or ablat ed to yield novel PIV vaccine candidates. Expression of the C, D and/or V ORF(s ) is reduced or ablated by modifying a recombinant PIV genome or antigenome, f or example by introduction of a stop codon, by a mutation in an RNA editing sit e, by a mutation that alters the amino acid specified by an initiation codon, o r by a frame shift mutation in the targeted ORF(s). Alternatively, the C, D and/or V ORF(s) is deleted in whole or in part to render the protein(s) encoded thereby partially or entirely non-functional or to disrupt protein expression altogether. C, D and/or V ORF(s) deletion and knock out mutants possess highly desirable phenotypic characteristics for vaccine development. These deletion and knock out mutations changes specify one or more desired phenotypic changes in the resulting virus or subviral particle. Vaccine candidates are generated that show a change in viral growth characteristics, attenuation, plaque size, and/or a change in cytopathogenicity, among other novel phenotypes. A variety of additional mutations and nucleotide modifications are provided within the C, D and/or V ORF(s) deletion or ablation mutant PIV of the invention to yield desired phenotypic and structural effects.
申请公布号 CA2378497(A1) 申请公布日期 2001.01.18
申请号 CA20002378497 申请日期 2000.07.06
申请人 THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC RET 发明人 COLLINS, PETER L.;MURPHY, BRIAN R.;DURBIN, ANNA P.
分类号 C12N15/09;A61K9/12;A61K39/00;A61K39/102;A61K48/00;A61P31/16;C07K14/115;C12N7/00;C12N7/04;(IPC1-7):C12N15/45;C12N15/11;A61K39/155 主分类号 C12N15/09
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