METHOD FOR SEPARATION AND PURIFICATION OF HIGH PURITY ROSMARINIC ACID FROM A PLANT BODY

摘要

PURPOSE: A method for separating and purifying rosmarinic acid as an antioxidant from a plant body, in particular the leaves of Perilla frutescens var. japonica Hara using water or a polar solvent is provided, which obtains high purity rosmarinic acid at low cost. CONSTITUTION: The rosmarinic acid of formula(I) as a natural antioxidant is obtained by extracting a plant body, in particular the leaves of Perilla frutescens var. japonica Hara in water or a polar solvent selected from ethyl acetate, acetone, methanol, ethanol, 1-propanol, 2-propanol, butanol, water and mixtures solvent thereof, removing volatile matter and a colorant with one of a nonpolar organic solvent and supercritical carbon dioxide and purifying using nonionic artificial synthetic absorption resins or gel, wherein the volatile matter and the nonpolar organic solvent for removing the colorant are selected from volatile hydrocarbons, chlorine-based organic solvents and mixture solvents thereof.