SOMATIC CELL-CLONED ANIMAL AND PREPARATION METHOD THEREOF

摘要

PURPOSE: A cloned animal, especially a clone calf and a preparation method thereof are provided which is capable of a high level of an organ or a medicine produced by the clone animal. CONSTITUTION: The preparation method of the cloned calf comprises the steps of : (a) obtaining and culturing the immature oocyte from an ovary of the slaughtered cattle; (b) preserving a cell line isolated from the variety of tissues; (c) removing 10-30 % of the a cytoplasm by cutting part of the oolemma of the mature oocyte of the step (a) to enucleate the oocyte; (d) injecting the donor cell line of the step (b) into the enucleated oocyte of the step (c); (e) electrofusing the designed oocyte from the step (d) at a voltage under DC condition; (f) activating the fused cell in a medium containing chemical; (g) culturing in a postactivation medium and a micro-droplet medium the activated cell and assaying the oocyte.