New nucleic acid encoding malic enzyme, useful for increasing yield of L-lysine in fermentation of transformed cells

摘要

Nucleic acid (I) having a sequence at least 50% identical with the gene encoding the malic enzyme (ME) of Corynebacterium glutamicum, is new. Independent claims are also included for the following: (a) polypeptide (II) with a sequence at least 75% identical with that of ME of C. glutamicum; (b) expression vector comprising a sequence (Ia) encoding an ME fused to a promoter functional in prokaryotic and/or eukaryotic cells, preferably C. glutamicum; (c) cells transformed with the vector of (b); and (d) production of L-lysine in cells that overexpress ME.