CONTINUOUS CARDIAC PERFUSION PRESERVATION WITH PEG-HB FOR IMPROVED HYPOTHERMIC STORAGE

摘要

The proposed use of the invention is for the ex vivo preservation of human and animal donor organ allografts during transportation from the donor to the recipient for the purpose of transplantation. In addition to its use for ex vivo myocardial preservation, this PEG-Hb solution has tremendous potential utility for in vivo myocardial preservation during open heart surgery as well as a blood substitute or blood replacement during or following surgery of any sort, including open heart surgery. The invention comprises a polyethylene glycol coated bovine hemoglobin based solution for the purpose of ex vivo donor organ preservation and the use of the same. The fundamental principle of the solution is to provide an oxygen, nutritional and electrolyte environment to the tissue of the donor organ that is conducive to ex vivo preservation such that the donor organ will regain acceptable function post transplantation. The solution provides oxygen, a carbohydrate energy source, continuous metabolite washout and continuous perfusion with an isotonic, normokalemic, hypocalcemic solution that drastically improves myocardial preservation over current techniques considered the standard of care. Donor organ preservation for transplantation is performed using ischemic hypothermic immersion storage in saline solution using hypothermic perfusion preservation with an oxygen carrying hemoglobin solution. The solution contains PEG-Hb, human albumin, dextrose, heparin sodium, lidocaine HC1, MgSO4, KC1, CaC12, THAM, NaC1, NaHCO3, Na3PO4, without which PEG-Hb is lethal to the myocardium and cannot be used for the purpose of effective organ preservation.