A gene encoding a protein constituting sucrose PTS of a coryneform bacterium provided by amplifying the downstream domain of a coryneform bacterium sucrase gene by the cassette-ligation mediated PCR method and thus obtaining a DNA encoding sucrose PTS enzyme II which is the protein (A) or (B) described below: (A) a protein having the amino acid sequence represented by SEQ ID NO:2 in Sequence Listing; and (B) a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:2 in Sequence Listing by substitution, deletion, insertion, addition or inversion of one or more amino acids and having an activity of binding to sucrose.
