The present invention provides a screening method for the identification of compounds which modulate an AMP-sensitive regulatory site on mitochondria comprising the steps of: a) contacting a test compound with mitochondria in the presence of a substrate for respiration in the presence of a buffer system; b) measuring an index of metabolic rate; and c) identifying compounds which modulate metabolic rate. Alternatively or additionally membrane potential can be measured. The invention also comprises a regulatory site for a mitochondrial proton leak which is related to the adenine nucleotide carrier, a binding assay and a functional assay.
申请公布号
WO0102598(A2)
申请公布日期
2001.01.11
申请号
WO2000EP05863
申请日期
2000.06.23
申请人
KNOLL AKTIENGESELLSCHAFT;BRAND, MARTIN, DENE;CADENAS, SUSANA;DICKINSON, KEITH;JONES, ROBERT, BRIAN
发明人
BRAND, MARTIN, DENE;CADENAS, SUSANA;DICKINSON, KEITH;JONES, ROBERT, BRIAN