L-lysine producing coryneform bacteria and methods for the production of L-lysine

摘要

L-lysine producing coryneform bacterium (A) with an amplified lysE (lysine exporter-carrier) gene in which at least one of the additional genes dapA (dihydropicolinate synthase), lysC (aspartate kinase), pyc (pyruvate carboxylase) and/or dapB (dihydropicolinate reductase) is amplified, preferably overexpressed. Independent claims are also included for the following: (a) DNA (I), replicable in coryneform bacteria and preferably recombinant DNA of Corynebacterium origin, which includes a sequence (1; 795 bp), reproduced, that represents the 5'-region of the dapB gene; (b) production of L-lysine by fermenting (A); Escherichia coli K12 strains DH5 alpha /pECTlysE (DSM 12871) or DH5 alpha /pEC7dapBlysE (DSM 12875); and Corynebacterium glutamicum strains DSM5715/pJC23 (DSM 12869), DSM5715aecD::dapA(MA16) (DSM 12867), DSM5715aecD::dapA(MC20) (DSM12868) and DM678 (DSM 12866).