| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new TSHR (Tag-TSHR) having high purity which consists of thyroid-stimulating hormone receptor(TSHR) whose N- or C-terminus is fused with a peptide and is useful for developing a medicine for treating Basedow's disease, and the like. SOLUTION: This is a new TSHR (Tag-TSHR) having high purity which consists of thyroid-stimulating hormone receptor(TSHR) and is useful in the field of fundamental studies such as the elucidation of epitope in TSHR which is recognized with antiTSHR autoantibody and the establishment of a TSHR- reacting T-lymphocyte and for developing a medicine for treating Basedow's disease and so on, wherein N- or C-terminus of the receptor(TSHR) is fused with a peptide. This Tag-TSHR is obtained by extracting mRNA from a human thyroid cell, preparing a cDNA library by the conventional method, screening the obtained library with antiTSHR antibody to give a gene coding for TSHR, linking myc peptide gene to signal peptide region at the end of the gene, incorporating the gene into an expression vector, transducing the obtained substance into CHO cell line to transform it, followed by culturing the obtained transformant. |
