| 摘要 |
PROBLEM TO BE SOLVED: To obtain a novel promoter which comprises a DNA molecule containing a specific base sequence and exhibiting a specific promoter activity and is used for the production,or the like, of a gene recombinant vaccine e.g. a recombinant virus, or the like, that has a short necessary domain and is highly active, safe and stable. SOLUTION: This promoter is a novel promoter comprising a DNA molecule containing the base sequence represented by formula I or a DNA molecule which is homologous to the DNA molecule of formula I but not homologous to the DNA represented by formula II and exhibits promoter activity and is useful for the production, or the like, of a gene recombinant vaccine that has a short necessary domain, shows high activity, safety and stability and contains a recombinant virus as a main ingredient. The promoter is obtained by separating a chicken genome to prepare a genomic bank by a conventional method, using this genomic bank as a template, preparing a DNA fragment containing a β-actin promoter by PCR using a primer comprising the partial sequence and removing more than 650 bp from the 3'-terminal side of the DNA fragment. |
