METHOD FOR PRODUCING CLONED COWS

摘要

The present invention provides a method for producing cloned cows by employing in vitro maturation of oocyte and nuclear transfer techniques. The method for producing cloned cows of the invention comprises the steps of: preparing donor somatic cells lines collected from cow; maturing oocytes collected from ovary in vitro; removing the cumulus cells surrounding the oocytes; cutting a portion of zona pellucida of the matured oocytes to make a slit, and squeezing out a portion of cytoplasm including the first polar body through the slit to give enucleated recipient oocytes; transferring a nucleus to the recipient oocyte by injection of the donor cells to the enucleated recipient oocytes, followed by the subseuent electrofusion and activation of the electrofused cells to give embryos; postactivating and culturing the embryos in vitro; and, transferring the cultured embryos into surrogate cows to produce cloned calves. The cloned cows can be employed to produce pharmaceuticals or organs, which facilitates their universal uses in medical and livestock industry, and scientific studies as well.