EXTRACTION OF INTEGRAL MEMBRANE PROTEINS

摘要

A process is described for extracting gram-negative integral membrane protei ns from bacteria or bacterial host cells containing a recombinant vector by differential detergent tangential flow diafiltration. This process has sever al advantages over alternate processes. First, it combines the clarification an d extraction processes into one unit operation. The product is extracted from the cells and it is separated from cell debris with only one continuous diafiltration process. Second, the membrane proteins are extracted in a semi - purified state, which simplifies the downstream processing steps. Third, thi s process is very scalable because the only requirement is that the surface ar ea of the membranes be increased proportionally with the amount of cells.