MOLECULAR SWITCHES

摘要

A method of selecting a switching system, the switching system comprising: (i) a first component comprising a first molecule and (ii) a second component comprising a second molecule, in which the first molecule binds to the second molecule in a manner modulatable by a ligand, and (iii) a third component comprising the ligand, the method comprising the steps of: (a) contacting one or more candidate first molecules with one or more candidate second molecules in the presence of one or more ligands; (b) selecting a complex of the three components; (c) optionally isolating and/or identifying the unknown components of the complex; (d) comparing the binding of the first molecule component of the complex to the second molecule component of the complex in the presence and absence of the ligand component of the complex; and (e) selecting complexes where said binding differs in the presence and absence of the ligand component, in which at least one component is provided in the form of a library of members.