| 摘要 |
<p>A fluorescent cyanine dye of the following general formula is disclosed: <CHEM> wherein: X1 and X2 are indipendently selected from the group consisting of -O-, -S-, -C(CH3)2 or -C=CH2; Y1 and Y2 are nonmetal atoms required to form a benzo-condensed or naphtho-condensed ring; Q is a conjugated moiety that increases the fluorescent quantum yield and the stability of the compound; R1 and R2 are indipendently selected from the group consisting of H, C1-C4, alkyl, alkylensulfonic group or alkylensulfonate group wherein the alkylene group has from 1 to 4 carbon atoms; R3, R4 and R5 are indipendently selected from the group consisting of H, a sulfonic group, a sulfonate group, alkylensulfonic, alkylensulfonate and -SO2NH(CH2)m-W-CH2)nZ, wherein alkylene has 1 to 4 carbon atoms, with the proviso that at least one of R1 to R5 contains a sulfonic or sulfonate group; W is absent or is a group selected from -SO2NH, -O-, -COO-, or -CONH-; n = 0-12 and m = 0-12 with the provisoes that m+n ≤ 12 and at least one of m and n NOTEQUAL 0; and Z is, or contains a N, O or S nucleophile functionality or is, or contains a functionality capable of reacting with N, O or S nucleophiles. Nucleophile functionalities include -NH2, -OH, and -SH groups; groups capable of reacting with such functionalities include -COCI, -COOCOR, -CONHNH2, N-hydroxysuccinimido esters, -NCS, -CHO, -COCH2I, phosphoramidite and maleimido. <IMAGE></p> |
