发明名称 |
Expression of G protein coupled receptors in yeast |
摘要 |
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein alpha subunit (mammalian Galpha). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein alpha-subunit (yeast Galpha) The cells are useful for screening compounds which affect the rate of dissociation of Galpha from Gbetagamma in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.
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申请公布号 |
US6168927(B1) |
申请公布日期 |
2001.01.02 |
申请号 |
US19980056920 |
申请日期 |
1998.04.08 |
申请人 |
DUKE UNIVERSITY |
发明人 |
KING KLIM;DOHLMAN HENRIK G.;CARON MARC G.;LEFKOWITZ ROBERT J. |
分类号 |
C07K14/39;C07K14/47;C07K14/705;C12N1/18;C12N1/19;C12N15/09;C12N15/12;C12N15/74;C12N15/81;C12P21/02;C12Q1/00;C12Q1/02;C12Q1/68;C12R1/865;G01N33/566;(IPC1-7):G01N33/53 |
主分类号 |
C07K14/39 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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