| 摘要 |
The invention relates to a method and device for separation of mixtures of molecules, especially mixtures of peptides and/or proteins, into components by gel electrophoresis in which the gel is reversibly coupled to a support, especially a support film. Preferably the gel is a polyacrylamide gel. Following the electrophoretic separation the gel, especially defined regions of the gel, is detached from the support by decoupling covalent bondings between gel and support. Then the gel, especially the defined regions, is removed in order to perform further analysis. In a particular embodiment of the invention the gel is an ultra thin gel of large dimensions. The inventive method is excellently suited for automatisation, high throughput and high resolution protein analysis. |
