A vector capable of expressing two foreign genes by using RRE sequence and controlling the ratio of the expression doses of these genes owing to the modification. This vector, which can be provided as a lentivirus vector based on SIV, is constructed by modifying a virus-origin expression regulatory sequence into another expression regulatory sequence so as to eliminate the dependency on the virus-origin protein. Although this vector has a packaging signal, it has been modified so that the risk of the occurrence of wild strains due to gene recombination is lowered and no virus structural protein is expressed. This vector is highly useful as a gene therapeutic vector with a need for transferring two genes while controlling the expression doses or expression dose ratio thereof.
申请公布号
WO0078987(A1)
申请公布日期
2000.12.28
申请号
WO2000JP03955
申请日期
2000.06.16
申请人
DNAVEC RESEARCH INC.;NAKAJIMA, TOSHIHIRO;NAKAMARU, KENJI;HASEGAWA, MAMORU;HAYAMI, MASANORI;IDO, EIJI