CHEMILUMINESCENCE REDOX ASSAY TO QUANTIFY ANALYTES IN BIOLOGICAL SAMPLES

摘要

A method for determining concentration of an analyte in a biological sample comprising the steps of: (a) combining the biological sample, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide (hereinafter NAD<+>), and a chemiluminescent label to form a reaction mixture; (b) allowing the analyte to undergo an oxidation-reduction reaction and NAD<+> to be converted to the reduced form of nicotinamide adenine dinucleotide (hereinafter NADH) and further allowing the chemiluminescent label to react with NADH; and (c) determining the concentration of the analyte of interest in the biological sample by correlating the quantity of light emitted with the concentration of NADH.