Recombinant fusion protein, (vaccine) composition containing the same and methodfor the production thereof

摘要

A fusion protein (I) comprising a fragment of Shiga toxin 2e (Stx2e) fused to a terminal tag whose size is no greater than that of the Stx2e fragment, is new. Independent claims are also included for the following: (1) a vaccine (II) comprising (I) for various uses in animal diseases associated with edema; (2) a plasmid (III) comprising DNA encoding (I); (3) an Escherichia coli strain transformed with (III); (4) production of (I) by cloning a subunit of the Stx2e operon in a vector system, transforming an Escherichia coli strain with the resulting recombinant plasmid, inducing the resulting expression system, and expressing and purifying the fusion protein; and (5) production of hybridoma clones for producing anti-Stx2eB immunoglobulins by fusing spleen cells from mice immunized with (I) with myeloma cells.