| 摘要 |
DNA constructs and methods for targeted integration of heterologous genes into a genome are disclosed. A transposon/transposase system is used to generate the substrates for homologous recombination in transgenic organisms. Targeted integration is selected in progeny using a combination of positive and negative selection markers. By eliminating the negative selection, the constructs and methods are adaptable for performing activation tagging, which relies on random integration events. |
